To East Brighton

Trams commence from Swanston Street near Faraday Street (University of Melbourne) and run via Swanston Street (City), St Kilda Road (Domain Interchange), Dandenong Road, and Hawthorn Road to terminate near Nepean Highway (Brighton East).

To Melbourne Uni

Trams commence at Hawthorn Road near Nepean Highway (Brighton East) and run via Hawthorn Road, Dandenong Road, St Kilda Road (Domain Interchange), Swanston Street (City) to terminate near Faraday Street (University of Melbourne).